Part:BBa_K643001:Design

Blue Light Promoter controlling Chloramphenicol Resistance

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The use of the psB1A3 as opposed to the psB1C3 backbone: The device produces chloramphenicol resistance in the cells. The backbone pSB1C3 has chloramphenicol resistance on its own and, if used as our backbone, would be redundant. It would not be possible to screen which cells were expressing the blue light promoter because chloramphenicol resistance would not be a guaranteed indicator of BLP expression.

Source

Blue Light Promoter BBa_K238013 From K.U. Leuven 2009

Chloramphenicol Resistance BBa_P1004 From Knight Lab, MIT 2006